An Improved Method For Silver Staining
While at MIT, Lennart and Robert Fink developed
a couple of modified Nauta  methods,
which have generally been known as the Fink-Heimer methods. The
use of the silver methods for the tracing of neuronal connections
is based on the fact that axons of neurons which are damaged by
a lesion brake up into fragments as they degenerate. The fragmented
axons can be visualized and distinguished from the multitude of
normal axons as they run through the tissue. The Fink-Heimer methods
were widely used in the late sixties, but were gradually replaced
by tracer techniques based on the principle of axoplasmic flow during
the seventies.
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